The project is taking place at Whitechapel station ahead of the arrival of Crossrail services in 2018.
A significant amount of development has already taken place at to rebuild the station and create new tunnels, however some train service suspensions are now required as the worksite is directly above London Overground tracks.
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As a result, the East London line is now partially closed until Sunday, 21 February.
Once work is complete, new lifts from street level to all train platforms will make the station fully accessible for customers using Crossrail, Tube or London Overground services. The ticket hall will also be refurbished and expanded with wider pathways and more ticket gates.
Mike Stubbs, TfL's Director of London Overground, said: "We have worked closely with our colleagues at Crossrail Limited to ensure that the work impacts on as few journeys as possible and take place during a quieter period on the network, when many schools are on their half term break."
The scheme is due to be completed in late 2018.
(LM)
