The firm was appointed as preferred supply chain partner under the ProCure21+ framework to support the project at Luton and Dunstable University Hospital.
The scheme is to be delivered by the firm's Northern Home Counties division.
The project includes a new £80m 'Hot Block' for all critical care, surgery and other complex care procedures.
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Other works include refurbishing several wards, delivering a new energy centre for the site and extending/refurbishing the Emergency Department.
Public facilities are to also be improved, including expanding the car park.
Work is due to begin to build a new £6m office block which is required to enable the delivery of the main works.
Peter Truscott, Chief Executive at Galliford Try, said: "This prestigious scheme is one of the largest on the ProCure21+ framework and is a fantastic win for our business. Galliford Try has a growing presence in the healthcare market and this is an excellent opportunity to demonstrate our capability in delivering complex public sector healthcare projects."
(LM/CD)
